Isolated heart studies

  • Isolated heart studies
  • Isolated heart studies
  • Isolated heart studies

Isolated heart experiments in Langendorff perfusion mode

How does it work?

In the Langendorff heart perfusion mode, the perfusate is pumped through the aorta towards the heart.
It does not enter the left ventricle, but is diverted into the coronary arteries.

The Langendorff heart mode permits the study of heart contraction, heart rate and vascular effects.

 
Advantages of the technique:

  • robust
  • rapid
  • cited in over 80,000 peer-reviewed scientific publications

  

Applications in Langendorff perfusion mode:

In Langendorff perfusion mode, the following parameters can be obtained:

  • intraventricular pressure (diastolic, systolic, developed pressure),
  • cardiac contractility and relaxation (dp/dt max and dp/dt min),
  • ECG
  • coronary flow

 

Isolated heart apparatus in Langendorff perfusion mode is commonly used for the study of:

  • drug-induced cardiac toxicity
  • ECG conductivity

Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart - Bialy et al - 2014

Integration of Multiple Cardiac Parameters to Predict Drug-Induced Cardiac Toxicity - Poster presented by Battelle at the Safety Pharmacology Society, Phoenix, AS, USA, October 2012

Whole Heart Energetics and Stress Test as an Indicator of Drug Induced Cardiac Toxicity - Poster presented by Battelle at the Society of Toxicology meeting 2013, San Antonio, TX, USA, March 2013

Increased Beta2-Adrenoceptors in Doxorubicin-Induced Cardiomyopathy in Rat - Nolwenn Merlet et al. 2013

 

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Isolated heart experiments in working heart perfusion mode

How does it work?

In the working heart perfusion mode, the flow of perfusate mimics the flow of blood in situ.

The perfusate enters the left atrium via the pulmonary vein, is pumped into the left ventricle, from where it is pumped into the aorta.

As the name implies, this technique allows the heart to perform its physiological pumping action, i.e. it performs pressure/volume work. The working heart technique therefore provides a complete analysis of heart function.

Advantages of the technique:

  • cardiac function correctly evaluated
  • robust, when surgery is correctly mastered


Applications in Working Heart mode:

In Working Heart perfusion mode, the following parameters can be obtained:

  • intraventricular pressure (diastolic, systolic, developed pressure),
  • cardiac contractility and relaxation (dp/dt max and dp/dt min),
  • cardiac cycle time (isovolumic contraction time, ejection time, isovolumic relaxation time, filling time)
  • ECG
  • PV loop
  • cardiac flow (coronary and aortic)

Isolated heart apparatus in Working Heart perfusion mode can be used for the study of:

  • cardiac metabolism
  • mitochondrial function
  • cell suffering
  • enzyme function
  • long-term pathology (ex: study of the ischemia-reperfusion on senescent heart)
  • ischemia-reperfusion (ex: study of postconditioning impact on cardiac function, oxidative stress, occurrence of arrhythmias and cellular suffering)

 

Metabolic effects of glutamine on the heart: Anaplerosis versus the hexosamine biosynthetic pathway - Benjamin Lauzier et al. Journal of Molecular and Cellular Cardiology (2013) 

Cardiac response to β-adrenoceptor stimulation is partially dependent on mitochondrial calcium uniporter activity. E Fernández-Sada et al. British Journal of Pharmacology (2014)

Ivabradine reduces heart rate while preserving metabolic fluxes and energy status of healthy normoxic working hearts. Benjamin Lauzier et al. Am J Physiol Heart Circ Physiol (2011)


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